| Position | Name | Age | Birthday | Nationality |
|---|---|---|---|---|
| Goalkeeper | Adam Zadražil | 24 | 2000-08-06 | - |
| Defender | Adam Čihák | 29 | 1995-04-13 | - |
| Midfielder | Adam Vlkanova | 30 | 1994-09-04 | - |
| Midfielder | Carlos Eduardo Lopes Cruz | 27 | 1997-08-08 | Brazil |
| Goalkeeper | Dominik Sváček | 27 | 1997-02-24 | - |
| Midfielder | Erik Jirka | 27 | 1997-09-19 | Slovakia |
| Midfielder | Jan Kopic | 34 | 1990-06-04 | - |
| Midfielder | Jan Sýkora | 30 | 1993-12-29 | - |
| Attacker | Jan Kliment | 31 | 1993-09-01 | - |
| Goalkeeper | Jindřich Staněk | 28 | 1996-04-27 | - |
| Midfielder | John Edison Mosquera Rebolledo | 34 | 1990-05-08 | Colombia |
| Defender | Josef Koželuh | 22 | 2002-02-15 | - |
| Defender | Libor Holík | 26 | 1998-05-12 | - |
| Defender | Luděk Pernica | 34 | 1990-06-16 | - |
| Defender | Lukáš Hejda | 34 | 1990-03-09 | - |
| Midfielder | Lukáš Kalvach | 29 | 1995-07-19 | - |
| Attacker | Lukáš Matějka | 26 | 1997-12-20 | - |
| Goalkeeper | Marián Tvrdoň | 30 | 1994-08-18 | Slovakia |
| Midfielder | Marko Alvir | 30 | 1994-04-19 | Croatia |
| Goalkeeper | Martin Jedlička | 26 | 1998-01-24 | - |
| Attacker | Matěj Vydra | 32 | 1992-05-01 | - |
| Defender | Milan Havel | 30 | 1994-08-07 | - |
| Midfielder | Modou Birame N'diaye | 28 | 1996-10-29 | Senegal |
| Defender | Mohamed Tijani | 27 | 1997-07-10 | Ivory Coast |
| Goalkeeper | Ondrej Mrózek | 21 | 2003-10-16 | - |
| Midfielder | Pavel Bucha | 26 | 1998-03-11 | - |
| Midfielder | Pavel Gaszczyk | 19 | 2005-02-17 | - |
| Attacker | Pavel Hašek | 19 | 2005-01-22 | - |
| Defender | Radim Řezník | 35 | 1989-01-20 | - |
| Defender | Robin Hranáč | 24 | 2000-01-29 | - |
| Midfielder | Roman Květ | 26 | 1997-12-17 | - |
| Attacker | Tomáš Chorý | 29 | 1995-01-26 | - |
| Goalkeeper | Viktor Baier | 19 | 2005-01-16 | - |
| Defender | Václav Jemelka | 29 | 1995-06-23 | - |
| Midfielder | Václav Pilař | 36 | 1988-10-13 | - |